| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.046478 |
| Chromosome: | chromosome 5 |
| Location: | 3445228 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g241350 | (1 of 1) 3.6.3.36 - Taurine-transporting ATPase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATGAATGTAACCCCACTCCCGCCGCCGC |
| Internal bar code: | CTTTGGGAGAGGCAGATCAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 447 |
| LEAP-Seq percent confirming: | 80.7428 |
| LEAP-Seq n confirming: | 587 |
| LEAP-Seq n nonconfirming: | 140 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCAGCATCTCAGAAGGAT |
| Suggested primer 2: | GGAAGAAGGGAGAGAAGCGT |