| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.046487 |
| Chromosome: | chromosome 4 |
| Location: | 1739417 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g212350 | (1 of 2) PF10013 - Uncharacterized protein conserved in bacteria (DUF2256) (DUF2256) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTACCTAAGGAGGCGTCAGGCGCGCACGT |
| Internal bar code: | TGCAGGTATCAGCTTGTTCGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 47 |
| LEAP-Seq percent confirming: | 61.1111 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACCTGCCCACAAAGGTAA |
| Suggested primer 2: | ACTGAACCAACCGTTTACGC |