Insertion junction: LMJ.RY0402.046504_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.046504
Chromosome:chromosome 5
Location:845979
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre05.g247850 (1 of 3) 3.6.1.22//3.6.1.55 - NAD(+) diphosphatase / NADP pyrophosphatase // 8-oxo-dGTP diphosphatase / 8-oxo-dGTPase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGGTTAGCGGGCACGGGTTGACCTGGCCA
Internal bar code:CATCGATGCCGTCGAACGGGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:702
LEAP-Seq percent confirming:96.8901
LEAP-Seq n confirming:48353
LEAP-Seq n nonconfirming:1552
LEAP-Seq n unique pos:141

Suggested primers for confirmation by PCR

Suggested primer 1:TATCTACAACCACGTGCCCA
Suggested primer 2:CATTGTCTTGCGCTCTGTGT