| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.046521 |
| Chromosome: | chromosome 9 |
| Location: | 3631027 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g390319 | (1 of 1) PTHR10663:SF42 - PROTEIN BOI2 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACTGGTCGGACATCGGATCTGCTTCGAC |
| Internal bar code: | ATCGCTACGGGGCGCGGAGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 280 |
| LEAP-Seq percent confirming: | 67.4877 |
| LEAP-Seq n confirming: | 137 |
| LEAP-Seq n nonconfirming: | 66 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCGACGAGTACACAGACGC |
| Suggested primer 2: | CACACTGTTGATCACCTCGG |