| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.046528 |
| Chromosome: | chromosome 9 |
| Location: | 5474949 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g400219 | (1 of 32) 3.1.1.3 - Triacylglycerol lipase / Triglyceride lipase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTTGCCTGCTGGGTGACGGGCAGGCAGC |
| Internal bar code: | TGAGTTCGGAGGCTACCGGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 177 |
| LEAP-Seq percent confirming: | 77.0917 |
| LEAP-Seq n confirming: | 182126 |
| LEAP-Seq n nonconfirming: | 54120 |
| LEAP-Seq n unique pos: | 102 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGAAGTCGTCTGAGGAGG |
| Suggested primer 2: | CTAAGTGGCTACAGCCCTGG |