Insertion junction: LMJ.RY0402.046562_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.046562
Chromosome:chromosome_7
Location:5515357
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre07.g351050 VPS23 Subunit of the ESCRT-I complex antisense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):TGAGTAAAACGCGTGCTGCCTGATAATGAA
Internal bar code:GTAACATAACACGACGCTCGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:454
LEAP-Seq percent confirming:99.695
LEAP-Seq n confirming:9151
LEAP-Seq n nonconfirming:28
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GCTTGTCGTACCCATGTCCT
Suggested primer 2:CGTGTGTGCTGTTACCGACT