Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.046562 |
Chromosome: | chromosome_7 |
Location: | 5515357 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre07.g351050 | VPS23 | Subunit of the ESCRT-I complex | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGAGTAAAACGCGTGCTGCCTGATAATGAA |
Internal bar code: | GTAACATAACACGACGCTCGAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 454 |
LEAP-Seq percent confirming: | 99.695 |
LEAP-Seq n confirming: | 9151 |
LEAP-Seq n nonconfirming: | 28 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCTTGTCGTACCCATGTCCT |
Suggested primer 2: | CGTGTGTGCTGTTACCGACT |