| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.046569 |
| Chromosome: | chromosome 2 |
| Location: | 3841307 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095750 | ZEP1,ZXE1 | (1 of 1) 1.14.13.90 - Zeaxanthin epoxidase / Zea-epoxidase; Putative zeaxanthin epoxidase-like protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCAGGGTCTGCGAAGATGCCATGCACAC |
| Internal bar code: | GTCGCCGATCCAACAGCGGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 220 |
| LEAP-Seq percent confirming: | 60.1064 |
| LEAP-Seq n confirming: | 2486 |
| LEAP-Seq n nonconfirming: | 1650 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGATTAGTGTCCACACGCT |
| Suggested primer 2: | TGGAGGTGTGTGTGTGTGTG |