| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.046570 |
| Chromosome: | chromosome 4 |
| Location: | 1530008 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g213650 | (1 of 25) IPR000210//IPR011333 - BTB/POZ domain // POZ domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGTGCAGCTGCTGGCGGAGCTGAAGGAAT |
| Internal bar code: | GTTTTAGTGTGCTAAATCACTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 956 |
| LEAP-Seq percent confirming: | 99.6874 |
| LEAP-Seq n confirming: | 21049 |
| LEAP-Seq n nonconfirming: | 66 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGACGGGACAAAAGACAT |
| Suggested primer 2: | ACGCAGTAATCTGCCTCACC |