| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.046580 |
| Chromosome: | chromosome 2 |
| Location: | 1660535 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g085650 | (1 of 1) K03424 - TatD DNase family protein (tatD) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGCACCACTCACATGGGTAGGCCCGACT |
| Internal bar code: | AACGAGTCGCATAGTAGGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 217 |
| LEAP-Seq percent confirming: | 99.7679 |
| LEAP-Seq n confirming: | 7306 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCATGACTCTGTTGCTTGC |
| Suggested primer 2: | ATACCGGTACATGAGCGGAG |