Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.046583 |
Chromosome: | chromosome_6 |
Location: | 7020110 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre06.g296850 | FAP81 | Flagellar Associated Protein | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTGGGAGTGTGCAGTGACTTCTGGATCGCA |
Internal bar code: | CTTGTTGCGATCGGGTCCTGTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 606 |
LEAP-Seq percent confirming: | 99.8706 |
LEAP-Seq n confirming: | 772 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCAGGAAAGAACTCAGTCGG |
Suggested primer 2: | TACATTGTCTTCGCGCTTTG |