| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.046584 |
| Chromosome: | chromosome 3 |
| Location: | 1722235 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g153450 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAACTGGGGAGTTCAGCTCGGGTTAGCCA |
| Internal bar code: | CCCACGGTATCTGAGACAAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 51 |
| LEAP-Seq percent confirming: | 86.7924 |
| LEAP-Seq n confirming: | 46 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATCGTTAGCAAGGTGTGGG |
| Suggested primer 2: | TTCACCACTAATTCTGCCCC |