Insertion junction: LMJ.RY0402.046585_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.046585
Chromosome:chromosome 5
Location:600668
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre05.g232100 (1 of 17) IPR001305 - Heat shock protein DnaJ, cysteine-rich domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCATCACCGCGTCCCCCCCCACCTCCTCC
Internal bar code:GGGGAGTATCGCGAGGGCTTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:446
LEAP-Seq percent confirming:99.6935
LEAP-Seq n confirming:1301
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CGAGAGCGTGTCTTAGTCCC
Suggested primer 2:AGCCACACAGCAGTGACAAC