Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.046585 |
Chromosome: | chromosome 5 |
Location: | 600668 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre05.g232100 | (1 of 17) IPR001305 - Heat shock protein DnaJ, cysteine-rich domain | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCATCACCGCGTCCCCCCCCACCTCCTCC |
Internal bar code: | GGGGAGTATCGCGAGGGCTTAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 446 |
LEAP-Seq percent confirming: | 99.6935 |
LEAP-Seq n confirming: | 1301 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGAGAGCGTGTCTTAGTCCC |
Suggested primer 2: | AGCCACACAGCAGTGACAAC |