| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.046601 |
| Chromosome: | chromosome 10 |
| Location: | 4011288 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g449050 | (1 of 3) 2.4.1.68 - Glycoprotein 6-alpha-L-fucosyltransferase / Guanosine diphosphofucose-glycoprotein fucosyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGAAACCGCTAAGGCCAGTGCCTAGACCT |
| Internal bar code: | ATTCAGCGCAAGTGGTCGAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 630 |
| LEAP-Seq percent confirming: | 99.643 |
| LEAP-Seq n confirming: | 13677 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCCCTCGCTGTTTGTTAC |
| Suggested primer 2: | TTGCCTTCCGTTCCTCATAC |