| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.046616 |
| Chromosome: | chromosome 10 |
| Location: | 4919244 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g454850 | (1 of 6) PF07716 - Basic region leucine zipper (bZIP_2) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAAAGGCCACGCGGCTTGCTGCAACTACT |
| Internal bar code: | TATTTGGACGGGATATCTCCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 486 |
| LEAP-Seq percent confirming: | 65.9316 |
| LEAP-Seq n confirming: | 598 |
| LEAP-Seq n nonconfirming: | 309 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTTCAGTTGTGGTTGCTGC |
| Suggested primer 2: | GGATGCTACTCGTGTGGGTT |