| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.046616 |
| Chromosome: | chromosome 10 |
| Location: | 4919264 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g454850 | (1 of 6) PF07716 - Basic region leucine zipper (bZIP_2) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCTGAATGTTGAGCAGGGACACTTCTCA |
| Internal bar code: | TGCGATGAGGGCTGGAGATACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 477 |
| LEAP-Seq percent confirming: | 99.6025 |
| LEAP-Seq n confirming: | 3007 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATGCTACTCGTGTGGGTT |
| Suggested primer 2: | TCTTCAGTTGTGGTTGCTGC |