| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.046642 |
| Chromosome: | chromosome 10 |
| Location: | 5585564 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g459900 | PFH4,PHX16,P4H4 | Prolyl 4-hydroxylase 4; (1 of 5) PTHR10869//PTHR10869:SF55 - PROLYL 4-HYDROXYLASE ALPHA SUBUNIT // OXOGLUTARATE/IRON-DEPENDENT OXYGENASE | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAAGGGTGGGGCGTGCGGGGTACGCGGA |
| Internal bar code: | TCGTCGGACAAGCAGTCGGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 638 |
| LEAP-Seq percent confirming: | 99.611 |
| LEAP-Seq n confirming: | 8962 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGCTGATTACGATGCGAG |
| Suggested primer 2: | GTCTCCACCACAATCACACG |