Insertion junction: LMJ.RY0402.046651_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.046651
Chromosome:chromosome_12
Location:2116003
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre12.g509100 sense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):TCAACATGCACCATGTACATATGAGTGCGA
Internal bar code:AGGCTGATGCCGCGAAAACCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:1050
LEAP-Seq percent confirming:99.508
LEAP-Seq n confirming:809
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CAGCACTTCTAAAGCCCCTG
Suggested primer 2:CACGTCATGGGTTGAAACTG