| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.046655 |
| Chromosome: | chromosome 13 |
| Location: | 2432672 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g579734 | GLC1 | (1 of 1) 3.2.1.106 - Mannosyl-oligosaccharide glucosidase / Processing A-glucosidase I; Glucosidase 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGAGGGTTGTAGATACCAGCCCGAACTA |
| Internal bar code: | GAGATGTAGGCGCTTAGTTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 428 |
| LEAP-Seq percent confirming: | 98.7624 |
| LEAP-Seq n confirming: | 399 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCCAAATAGAAGTCCGCT |
| Suggested primer 2: | GTGTGCGAGGGAAAAGGTAA |