| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.046659 |
| Chromosome: | chromosome 12 |
| Location: | 3155610 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g499451 | (1 of 1) PTHR14089//PTHR14089:SF2 - CELL CYCLE CONTROL PROTEIN // PRE-MRNA-SPLICING FACTOR CWC2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTTTTACATCAGTGGTTTCCTCCATCTAC |
| Internal bar code: | TTCCGATGCCAATTTCGGTCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 789 |
| LEAP-Seq percent confirming: | 95.8363 |
| LEAP-Seq n confirming: | 1358 |
| LEAP-Seq n nonconfirming: | 59 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACGGCAAGGAAGCGTATC |
| Suggested primer 2: | AGAGAGGTTGGGGAAGGTGT |