Insertion junction: LMJ.RY0402.046684_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.046684
Chromosome:chromosome_7
Location:3543042
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre07.g336300 sense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):TTCACATGCCTTTCGTTCACATGTGCACTC
Internal bar code:CAAGTGCCAGACCCCCCGTCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:700
LEAP-Seq percent confirming:99.6319
LEAP-Seq n confirming:3248
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:CCGAACCCCGTATTTCCTAT
Suggested primer 2:TTGCCGTTGTTAGTGAGCAG