| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.046696 |
| Chromosome: | scaffold 36 |
| Location: | 15190 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre36.g759697 | (1 of 781) IPR000104 - Antifreeze protein, type I | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTTGGGACTGGTGGCGGGGAAGGGGAGA |
| Internal bar code: | ATTGCCCTCACATTGTCTTCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 895 |
| LEAP-Seq percent confirming: | 99.4432 |
| LEAP-Seq n confirming: | 12502 |
| LEAP-Seq n nonconfirming: | 70 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGCTACCAAAAAGAGCGG |
| Suggested primer 2: | TGTAGGTGTAGTTGCGGCAG |