| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.046703 |
| Chromosome: | chromosome 10 |
| Location: | 5666938 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g460532 | APC2 | (1 of 1) K03349 - anaphase-promoting complex subunit 2 (APC2); Anaphase promoting complex subunit 2 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTGAAGCGAACCGCCTTCCAATCGCCGC |
| Internal bar code: | CACAGGGGCAACGTATTTCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 137 |
| LEAP-Seq percent confirming: | 99.7859 |
| LEAP-Seq n confirming: | 466 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTGTGTGTGGGTGTGGAG |
| Suggested primer 2: | GTGTGTGAGGTGAGTGGGTG |