Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.046749 |
Chromosome: | chromosome_6 |
Location: | 6510837 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre06.g293150 | PGM12 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ATGACTCCATGGGGGTGAAGGGGCGCATGT |
Internal bar code: | GCCCGCAGCCAGATGAGAAGGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 335 |
LEAP-Seq percent confirming: | 97.4463 |
LEAP-Seq n confirming: | 3129 |
LEAP-Seq n nonconfirming: | 82 |
LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACAACACGGACCTGAACACA |
Suggested primer 2: | CTCCTTCTACTCCGCCTCCT |