| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.046749 |
| Chromosome: | chromosome 6 |
| Location: | 6510837 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g293150 | PGM12 | (1 of 2) PTHR23029:SF12 - PHOSPHOMUTASE PMU1-RELATED; Phosphoglycerate mutase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGACTCCATGGGGGTGAAGGGGCGCATGT |
| Internal bar code: | GCCCGCAGCCAGATGAGAAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 335 |
| LEAP-Seq percent confirming: | 97.4463 |
| LEAP-Seq n confirming: | 3129 |
| LEAP-Seq n nonconfirming: | 82 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAACACGGACCTGAACACA |
| Suggested primer 2: | CTCCTTCTACTCCGCCTCCT |