| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.046755 |
| Chromosome: | chromosome_1 |
| Location: | 3914102 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g025450 | FAP122,DRC9 | Flagellar Associated Protein | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTTGCTCCTGTCTCAGCGACGCAAGCCGCG |
| Internal bar code: | GGTTACGGGGTGATGCGAGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 626 |
| LEAP-Seq percent confirming: | 99.7392 |
| LEAP-Seq n confirming: | 765 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTGTAAGGGGTTCTGCCT |
| Suggested primer 2: | CTTGAAGCTGGCCAACTAGG |