| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.046777 |
| Chromosome: | chromosome 16 |
| Location: | 2057283 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g657200 | GOX17,GOX18 | (1 of 12) 1.1.3.9 - Galactose oxidase / Beta-galactose oxidase; Glyoxal oxidase 17 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCATAGCAGTCTTGTAGCAGTAATTGCGC |
| Internal bar code: | TGAGGGGACATGTCGTGGTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 313 |
| LEAP-Seq percent confirming: | 98.1132 |
| LEAP-Seq n confirming: | 260 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGGCCAATGTGCATAAGT |
| Suggested primer 2: | TACCGTGTTGAGCTGTTTGC |