| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.046781 |
| Chromosome: | chromosome 16 |
| Location: | 6108450 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g675850 | (1 of 1) K11729 - acyl-CoA dehydrogenase family member 10 (ACAD10) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAATGGTCGCGCAACCTACCGGCCTCGA |
| Internal bar code: | GACCGGGGGTGTGCCGGGACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 549 |
| LEAP-Seq percent confirming: | 99.6078 |
| LEAP-Seq n confirming: | 762 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGTGGGTGGATAGGCTT |
| Suggested primer 2: | TCTGTGATACGATTCGAGCG |