| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.046786 |
| Chromosome: | chromosome 6 |
| Location: | 2585840 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g269450 | EIF3G | Eukaryotic translation initiation factor 3, subunit G; (1 of 1) K03248 - translation initiation factor 3 subunit G (EIF3G) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAATGGACGCGTGAGGATGTGCCCGGCCAA |
| Internal bar code: | ACACGATGGCGGGATTAGAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 337 |
| LEAP-Seq percent confirming: | 74.2574 |
| LEAP-Seq n confirming: | 75 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGATGGTGTTGACAGGGGAG |
| Suggested primer 2: | CCCAGTTTACTGCGTGTCAA |