| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.046789 |
| Chromosome: | chromosome 17 |
| Location: | 1906963 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g710300 | PHC31 | (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 31 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATCCAGGGCTGCAGCAGGTTGGCGTCCTC |
| Internal bar code: | AAGCCAAAAAATAGTGCATGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 207 |
| LEAP-Seq percent confirming: | 99.8426 |
| LEAP-Seq n confirming: | 1903 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGATCTGCATGACCCTGGAC |
| Suggested primer 2: | GGTCCACCACTCAATCTTGG |