| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.046824 |
| Chromosome: | chromosome 6 |
| Location: | 3290109 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g277100 | (1 of 4) PF03435 - Saccharopine dehydrogenase NADP binding domain (Sacchrp_dh_NADP) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCACCGGCCGCAGGCCATCCCTCCGTCGT |
| Internal bar code: | GACATGTGAGACATCAGCCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 495 |
| LEAP-Seq percent confirming: | 99.0539 |
| LEAP-Seq n confirming: | 31304 |
| LEAP-Seq n nonconfirming: | 299 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGTTGCATACACAAGCAT |
| Suggested primer 2: | AAATTCACCCGCTTGCTCTA |