Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.046837 |
Chromosome: | chromosome_5 |
Location: | 1162804 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre05.g245550 | PIK1 | Phosphatidylinositol 4-kinase | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTGCATTTGCTGCAGGCCGTCACTGTTTGG |
Internal bar code: | GTTCTTTGATGTGAGATCTTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 292 |
LEAP-Seq percent confirming: | 99.3243 |
LEAP-Seq n confirming: | 294 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTGGCAGTGTTGGGCTTTAT |
Suggested primer 2: | GTGCGAGGTCATATTTCGGT |