Insertion junction: LMJ.RY0402.046851_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.046851
Chromosome:chromosome 6
Location:1114920
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g256800 SND1A SAND domain-encoding short ORF; (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATGTACGGTAGTCAAGGACGGATGCACAG
Internal bar code:TCCTCTCGGACGGTGTGGGGTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:937
LEAP-Seq percent confirming:99.6554
LEAP-Seq n confirming:1446
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:GGGGTAGCCACCTACCTAGC
Suggested primer 2:AACACGGTAGGTTCACGAGG