| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.046862 |
| Chromosome: | chromosome 7 |
| Location: | 2062420 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325850 | MAW8 | Membrane-associated hydroxyproline-rich glycoprotein 8 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGTTTGTGGGATCGCCGCGCAAATGCTCC |
| Internal bar code: | CCGTTTGGGAACAGCTCACATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 344 |
| LEAP-Seq percent confirming: | 99.2998 |
| LEAP-Seq n confirming: | 9927 |
| LEAP-Seq n nonconfirming: | 70 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGACGTTTGTGTTTGTGG |
| Suggested primer 2: | GGCCTTGGGGATCTAGTAGG |