| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.046923 |
| Chromosome: | chromosome 3 |
| Location: | 4379851 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g175400 | PGI,PGI1 | Phosphoglucose isomerase 1; (1 of 1) 5.3.1.9 - Glucose-6-phosphate isomerase / Phosphosaccharomutase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGGCTGGTCGCGCCTCGCACGCAAAGAC |
| Internal bar code: | GGGCGGACGGGCTCTAGAGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 632 |
| LEAP-Seq percent confirming: | 95.8102 |
| LEAP-Seq n confirming: | 2927 |
| LEAP-Seq n nonconfirming: | 128 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGTAGAGGTGTGGTGTCG |
| Suggested primer 2: | TTTTAGGCGTGGTTGGTAGC |