| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.046944 |
| Chromosome: | chromosome_4 |
| Location: | 2238749 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre04.g220076 | sense | gene_edge/mRNA_edge/3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GAGTACACCCAACGATCCAAAACGACTGCA |
| Internal bar code: | ACGCAGGCGCACTAACAGTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 353 |
| LEAP-Seq percent confirming: | 99.5893 |
| LEAP-Seq n confirming: | 485 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTGCTGTCTGTTGCATAA |
| Suggested primer 2: | CTGGTAATTGTTGAGCCCGT |