| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.046955 |
| Chromosome: | chromosome 12 |
| Location: | 4057623 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g517550 | RRM10 | Putative ribosomal RNA methyltransferase; (1 of 2) 2.1.1.137 - Arsenite methyltransferase / S-adenosyl-L-methionine:methylarsonite As-methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGGATGCCGTGCGCACCCCAGCCCGGCC |
| Internal bar code: | CACATATGTTTGTCGTTTACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 669 |
| LEAP-Seq percent confirming: | 98.9906 |
| LEAP-Seq n confirming: | 4315 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGCCACGTGATAGGTTTT |
| Suggested primer 2: | AGCTGAATCCATAAATGCGG |