| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.046974 |
| Chromosome: | chromosome 6 |
| Location: | 5058349 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g280475 | (1 of 1) PTHR13018//PTHR13018:SF40//PTHR13018:SF5 - PROBABLE MEMBRANE PROTEIN DUF221-RELATED // SUBFAMILY NOT NAMED // PHOSPHATE METABOLISM PROTEIN 7 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCAGTCGCCGTTCTATGCACCCGTGCCC |
| Internal bar code: | CTTAATGGTCGATTGTTGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 865 |
| LEAP-Seq percent confirming: | 99.636 |
| LEAP-Seq n confirming: | 2737 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACCGTGCCTAGAAACCCT |
| Suggested primer 2: | GCCCTCCTTCCAGATCCTAC |