Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.046984 |
Chromosome: | chromosome 6 |
Location: | 1437703 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre06.g259200 | EFS2,EFS1,SELB,SELB1 | Selenocysteine-specific elongation factor EF-Sec; (1 of 1) PTHR23115:SF91 - SELENOCYSTEINE-SPECIFIC ELONGATION FACTOR | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGCCGCCTTTCCTGGACGGCTACCCCCC |
Internal bar code: | GTTCAAGCGTACCCGTCGCTCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 128 |
LEAP-Seq percent confirming: | 99.5715 |
LEAP-Seq n confirming: | 1162 |
LEAP-Seq n nonconfirming: | 5 |
LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGGCACAAGCAACAAGTGTG |
Suggested primer 2: | CTCTTCAACGCCCAGTTCTC |