| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.046984 |
| Chromosome: | chromosome 6 |
| Location: | 1437703 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g259200 | EFS2,EFS1,SELB,SELB1 | Selenocysteine-specific elongation factor EF-Sec; (1 of 1) PTHR23115:SF91 - SELENOCYSTEINE-SPECIFIC ELONGATION FACTOR | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGATTGAAGGTGGAGGCGGAGTAGGCAT |
| Internal bar code: | ATATACGTTACTTCGGTCCTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 238 |
| LEAP-Seq percent confirming: | 99.3763 |
| LEAP-Seq n confirming: | 3824 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCACAAGCAACAAGTGTG |
| Suggested primer 2: | CTCTTCAACGCCCAGTTCTC |