Insertion junction: LMJ.RY0402.046986_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.046986
Chromosome:chromosome 2
Location:4021381
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g097100 (1 of 8) PF01697 - Glycosyltransferase family 92 (Glyco_transf_92) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGTCTGTCACAAACCCGTCACCCGCTACA
Internal bar code:TCATTATCGTGGATCAAAGGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:367
LEAP-Seq percent confirming:98.7578
LEAP-Seq n confirming:954
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GAGTACACGGAGCGTGTGAA
Suggested primer 2:TGTACGGTTCAATCATGCGT