| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.047008 |
| Chromosome: | chromosome 2 |
| Location: | 4921031 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g103650 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGGTTAGGCACAGGAACATATACGGTAT |
| Internal bar code: | GGGAGCCCTTTCCCTCCGGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 356 |
| LEAP-Seq percent confirming: | 96.2637 |
| LEAP-Seq n confirming: | 1752 |
| LEAP-Seq n nonconfirming: | 68 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACAGTCCGCATTGATGCTC |
| Suggested primer 2: | CCGTCGGATTATGTTCGTCT |