Insertion junction: LMJ.RY0402.047076_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.047076
Chromosome:chromosome 11
Location:3707711
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g482750 (1 of 239) IPR016024 - Armadillo-type fold 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTTCCCTCGCGAGCGTCTCCCTCAACTCT
Internal bar code:GCGTCGAGGCAGTGGGGCTTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:378
LEAP-Seq percent confirming:98.4747
LEAP-Seq n confirming:8199
LEAP-Seq n nonconfirming:127
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:GGAAACGTCTCAGGTGGTGT
Suggested primer 2:ACACACACACACACGCACAC