| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.047083 |
| Chromosome: | chromosome 5 |
| Location: | 2860384 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g237870 | 5'UTR | ||
| Cre05.g237890 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAGAGGAGGGGCAGGTGGACAGCGCGGT |
| Internal bar code: | AAGGGAATCTGAGAGCGGACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 483 |
| LEAP-Seq percent confirming: | 97.3118 |
| LEAP-Seq n confirming: | 1448 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGATACGGTACGGGGAAG |
| Suggested primer 2: | GCCACTCACAAGGAGAAAGC |