| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.047100 |
| Chromosome: | chromosome 6 |
| Location: | 8684612 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g309650 | (1 of 1) IPR003200//IPR006626//IPR011050 - Nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase-like // Parallel beta-helix repeat // Pectin lyase fold/virulence factor | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGCGGTGGCCTCAGCTGCCTCCCTACGC |
| Internal bar code: | AGTGGTTGGTTGTGGGGTGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 295 |
| LEAP-Seq percent confirming: | 99.8318 |
| LEAP-Seq n confirming: | 2374 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGGCCCTAGTGTGTACGTC |
| Suggested primer 2: | CTGTTGTCATTCATCCTGCG |