| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.047131 |
| Chromosome: | chromosome 12 |
| Location: | 4029768 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g517150 | MET16,APSR1,APR1 | Adenylylphosphosulfate reductase; (1 of 1) 1.8.99.2 - Adenylyl-sulfate reductase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTAGTAATGCCCCGGCCGCACCTGGCAGA |
| Internal bar code: | AAATTCTTGAGGTGCACTAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1121 |
| LEAP-Seq percent confirming: | 99.8026 |
| LEAP-Seq n confirming: | 1011 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACAGCCTACTCAGCCTGGA |
| Suggested primer 2: | CACAAGAGCTGCACACGAAT |