| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.047141 |
| Chromosome: | chromosome 11 |
| Location: | 2446814 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g475400 | (1 of 1) K11864 - BRCA1/BRCA2-containing complex subunit 3 (BRCC3) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGCGGATAGAAACAGGGGGCACGGTGTA |
| Internal bar code: | GTTCTTGAAGGTGTAATTCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 339 |
| LEAP-Seq percent confirming: | 99.8449 |
| LEAP-Seq n confirming: | 2575 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGTGCATTGAAGCTGACGG |
| Suggested primer 2: | CTGGAGTGTACCAGGTCCGT |