Insertion junction: LMJ.RY0402.047175_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.047175
Chromosome:chromosome 12
Location:9534451
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g544752 (1 of 26) IPR013026//IPR019734 - Tetratricopeptide repeat-containing domain // Tetratricopeptide repeat intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGAGCTGTTGGAAGAACAAGGCACATGCAG
Internal bar code:CTACGCCGCTCCCCAAAGGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:883
LEAP-Seq percent confirming:95.4556
LEAP-Seq n confirming:8276
LEAP-Seq n nonconfirming:394
LEAP-Seq n unique pos:79

Suggested primers for confirmation by PCR

Suggested primer 1:AAGGAGCTGCCACTGAACAT
Suggested primer 2:GACGGGTTGACTGGTTGAGT