| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.047178 |
| Chromosome: | chromosome 7 |
| Location: | 3644622 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g337125 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAAGGTGACCGATCCGACGTCCGCGTGAC |
| Internal bar code: | CCCCAACTACTCACTCTGGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 760 |
| LEAP-Seq percent confirming: | 99.5494 |
| LEAP-Seq n confirming: | 2651 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAGGACTTGACGTCAGCA |
| Suggested primer 2: | GAACAAGCTTCGCAAAGGAC |