| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.047179 |
| Chromosome: | chromosome 14 |
| Location: | 3011922 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g628100 | SPL7 | Putative hnRNP splicing factor | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTCGTAATGGAGTGTTAGGTGTGCAGAG |
| Internal bar code: | CGTCAGGACCCCAGCACCTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 986 |
| LEAP-Seq percent confirming: | 98.4694 |
| LEAP-Seq n confirming: | 965 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGGGTTGCCTGTTGTAAT |
| Suggested primer 2: | ATCCCCTACTCCCAATGTCC |