Insertion junction: LMJ.RY0402.047181_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.047181
Chromosome:chromosome 9
Location:6509369
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g407501 (1 of 5) 3.2.1.3 - Glucan 1,4-alpha-glucosidase / Lysosomal alpha-glucosidase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCGTGTTCCGAGTGGCTGTGCTGGTCGCC
Internal bar code:GCCTGCTATCGTTGTTCATCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:489
LEAP-Seq percent confirming:98.2637
LEAP-Seq n confirming:3622
LEAP-Seq n nonconfirming:64
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:CTCGTTTCTGCGGTTTTCTC
Suggested primer 2:TAGTGCTGGCGTCAAGAATG