Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.047181 |
Chromosome: | chromosome 9 |
Location: | 6509369 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre09.g407501 | (1 of 5) 3.2.1.3 - Glucan 1,4-alpha-glucosidase / Lysosomal alpha-glucosidase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGTGTTCCGAGTGGCTGTGCTGGTCGCC |
Internal bar code: | GCCTGCTATCGTTGTTCATCAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 489 |
LEAP-Seq percent confirming: | 98.2637 |
LEAP-Seq n confirming: | 3622 |
LEAP-Seq n nonconfirming: | 64 |
LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTCGTTTCTGCGGTTTTCTC |
Suggested primer 2: | TAGTGCTGGCGTCAAGAATG |