| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.047193 |
| Chromosome: | chromosome_6 |
| Location: | 6999633 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g296700 | HYDG1,HYDG | Hydrogenase assembly factor/biotin synthase | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCCGAGAAGATCCGTCCAAACGCGCGGACG |
| Internal bar code: | TCAAGCCTAAGGTTTTTGTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 963 |
| LEAP-Seq percent confirming: | 99.7634 |
| LEAP-Seq n confirming: | 5482 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATTCCAGTCCTCAAGCTGC |
| Suggested primer 2: | CAAAAATTGCAGCACGAGAA |